Molecular Genomics Core

Q: Who can use the Genomic Core services?

A: Our services are available to researchers within our institution as well as external academic and commercial entities.

Q: What types of library services does the Molecular Genomics Core offer?

A: The MGC offers a wide range of options, including DNA-seq libraries, RNA-seq libraries, and all types of 10x libraries.

DNA-seq libraries include:

1) Whole genome seq library

2) Whole exome seq library

3) ChIP seq library

4) Methyl-seq library

5) Hi-C library

6) Targeted capture

RNA-seq libraries include:

1) Total RNA-seq library

2) mRNA-seq library

3) MicroRNA-seq library

4) 16s, ITS amplicon-based libraries

5) Library for SAR-CoV-2 virus

10x assays include:

1) Single cell/nuclei Gene expression (3’ GEX)

2) Single cell Immune Profiling (5’GEX +DVJ)

3) Single nuclei ATAC-seq

4) Single nuclei Multiome (ATAC+ GEM)

5) 10x Visium gene expression

Q: Does the Molecular Genomics Core provide single cell/nuclei isolation services?

A: Yes. We strongly recommend that customers process single cell/nuclei assays independently. However, if needed, we are available to provide these services.

Q: Can the Molecular Genomics Core help with experimental design?

A: Absolutely. Our team can assist with experimental design to ensure optimal results and efficient use of resources.

Q: What is the turnaround time for sequencing services?

A: Turnaround times vary depending on the type and scope of the project. Typical turnaround times range from a few days to several weeks.

Q: How much do your services cost?

A: Pricing varies based on the specific services and project requirements. Please contact us for a detailed quote.

Q: Do you offer training or workshops on genomic technologies?

A: Yes, we offer regular training sessions and workshops on various genomic technologies. Check our website for upcoming events.

Q: Are you open to collaborative research projects?

A: We welcome collaborative research projects and are happy to discuss potential collaborations. Please contact us to explore opportunities.

Q: When is TPPC closed?

A: TPPC is open 24/7 and staffed 9 AM – 5 PM on weekdays. TPPC follows the TAMU holiday schedule for days off. 

Q: Where can I find the full list of services TPPC offers? 

A: Please visit Services and Shared Workspaces

NOTE: Services will be performed by staff from the beginning to the end. For shared workspace, you can either conduct the experiment on your own or request it to be conducted by staff for an extra fee. 

Q: How long does a service request take? 

A: It will depend on scheduling and reagent availability, but in general, 

• For Chemistry analysis with DxC 700 AU, Tissue processing with ASP300S, Staining with Spectra Stainer
  • If the sample is dropped off in the morning, results or pickup will be ready that afternoon 
  • If the sample is dropped off in the afternoon, results or pickup will likely be ready the next day in the afternoon 

• Cryosection with Leica CM 1950 depends on sample quantity; please contact TPPC@tamu.edu for estimates. 

Q: How do I request service or reserve shared workspace? 
A: For service requests: 

1. Create a valid iLab account:
   • Go to Texas A&M Preclinical Phenotyping Core iLab
   • Sign-up with TAMU or your institution’s credentials. 
   • Click the three-bar icon located in the upper left corner, to the left of the Agilent Cross Lab logo. 
   • Go to Manage Groups/My Groups. 
   • If your lab group is not listed under “My Groups,” click Request Group Access and follow the on-screen instructions. 
   • PI or Lab/Group Manager can accept your membership request and add a funding source. Instructions for PI/Manager here

2. Download the request forms: 
   • Blood and urine chemistry analysis with DxC 700 AU *link to download request form; file can be found in wordpress* 
   • Tissue processing with Leica ASP300S *link to download request form; file can be found in wordpress* 
   • Cryosection with Leica CM 1950 *link to download request form; file can be found in wordpress*. Leica CM 1950 Cryostat is also available for shared workspace. 
   • Slide Staining with Leica HistoCore Spectra Stainer and cover slipper 

3. Email the completed request form to TPPC@tamu.edu or TPPC staff to schedule the request. 

A: For Shared Workspaces: 

1. Confirm your AUP – All behavioral tests conducted in TPPC should be listed in your Animal Use Protocol (AUP) 

2. Create a valid iLab account: 
   • Go to Texas A&M Preclinical Phenotyping Core iLab
   • Sign-up with TAMU or your institution’s credentials. 
   • Click the three-bar icon located in the upper left corner, to the left of the Agilent Cross Lab logo. 
   • Go to Manage Groups/My Groups. 
   • If your lab group is not listed under “My Groups,” click Request Group Access and follow the on-screen instructions. 
   • PI or Lab/Group Manager can accept your membership request and add a funding source. Instructions for PI/Manager here

3. Get trained – Get a trained user status by either completing the training offered by TPPC or skipping the training if you already have experience using the instrument. Email TPPC@tamu.edu or TPPC staff to schedule training. 

4. Building access – Request building access by emailing to TPPC@tamu.edu if you are interested in using shared workspace in LARR. Shared workspace in REYN does not require building access if the reservation is between 8 AM to 5 PM on weekdays. If you are interested in using REYN shared workspace after hours or during the weekends, please also email TPPC@tamu.edu for further information. 

5. Schedule your reservation – Once your iLab account is set as a trained user, please go to Texas A&M Preclinical Phenotyping Core iLab and click Schedule Equipment tab to create reservations. 

Q: I have problems with iLab. 

A: Please email TPPC@tamu.edu.